Fourier transform infrared spectroscopic imaging identifies early biochemical markers of tissue damage

• DOI: 10.1117/12.2040282
• Publication Date: 2014-03-04T02:39:48Z
• Authors: Vishal K. Varma; Samuel Ohlander; Peter Nguyen; Christopher Vendryes; Sujeeth Parthiban; Blake Hamilton; M. Chad Wallis; Andre Kajdacsy-Balla; Blake Hannaford; Thomas Lendvay; James M. Hotaling; Michael J. Walsh
• Keywords: Fourier transform spectroscopy

Fourier Transform Infrared (FT-IR) spectroscopic imaging can allow for the rapid of tissue biochemistry in a label-free and non-perturbing fashion. With adoption new minimally invasive surgery (MIS) technologies over last 20 years, adequate skill to safely effectively use these may not be achieved risk undue physical pressure being placed on tissues is concern. Previous work has demonstrated that number histological stains detect damage, however, this process requires initiation progression signaling cascade results epitope interest expressed. We proposed identify early biochemical markers associated with damage from applied forces, thus requiring transcriptional translational protein synthesis as traditional immunohistochemistry does. To demonstrate FT-IR measure changes have undergone force, we took ex-vivo lamb's liver had been freshly excised varying levels (0kPa 30kPa). Tissues were then formalin-fixed, paraffin-embedded, sectioned glass H E staining an IR slide imaging. Regions containing hepatocytes identified average spectra extracted damaged undamaged livers. showed clear damage. In addition, chemical could observed proceeding when using conventional approaches.