Optimization of ultrasound contrast agent and treatment duration for drug delivery to methicillin-resistant Staphylococcus aureus diabetic wound biofilms in mice

• DOI: 10.1121/10.0027528
• Publication Date: 2024-07-03T13:30:14Z
• Authors: Kelly VanTreeck; Jamie Liu; Ashelyn Sidders; Kuan-Yi Lu; Amanda Velez; Phillip Durham; Duyen Bui; Michelle Angeles-...; Paul A. Dayton; Sarah Rowe; Virginie Papadopo...
• Keywords: 03 medical and health sciences; 0302 clinical medicine

Chronic wounds are frequently infected with bacterial biofilms which pose a barrier to drug diffusion, uptake, and facilitate tolerance, thereby prolonging the healing process in chronic increasing likelihood of relapse. A major contributor relapse is persister cells, subset resilient high antibiotic doses. Previous work by our group showcased significantly improved gentamicin efficacy diabetic murine wound model using ultrasound, nanodroplets, an anti-persister drug. Here, we aim optimize nanodroplet formulation for cell targeting treatment duration clinical translation. In methicillin-resistant Staphylococcus aureus (MRSA) SKH-1 hairless mice (n = 5), were treated twice daily gentamicin, nanodroplets containing oxygen or palmitoleic acid (PA). These components increase oxygenation enhance respectively. Additionally, treatments PA ultrasound 2, 5, 10 minutes investigated 3). Preliminary findings indicate most effectively potentiates activity, 10- minute improve 1-log compared 5-minutes, 2-minute achieve comparable 5-minutes. initial suggest promising strategy enhancing cells biofilms, consideration resource-efficient times application.