Real-time nanodiamond thermometry probing in vivo thermogenic responses

• DOI: 10.1126/sciadv.aba9636
• Publication Date: 2020-09-11T23:15:34Z
• Authors: Masazumi Fujiwara; Simo Sun; Alexander Dohms; Yushi Nishimura; Ken Suto; Yuka Takezawa; Keisuke Oshimi; Li Zhao; Nikola Sadzak; Yumi Umehara; Yoshio Teki; Naoki Komatsu; Oliver Benson; Yutaka Shikano; Eriko Kage-Nakadai
• Keywords: Nanodiamond

Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities, such as homeostatic thermoregulation and energy metabolism. However, it is challenging to reduce the size bio-compatible thermometers down submicrometers despite potential applications for thermal imaging subtissue structures with single-cell resolution. Light-emitting nanothermometers that remotely sense via optical signals exhibit considerable in \textit{in-vivo} high-spatial-resolution thermometry. Here, using quantum based on optically accessible electron spins nanodiamonds (NDs), we demonstrate real-time \textit{Caenorhabditis elegans} (\textit{C. elegans}) worms. We developed thermometry system can temperatures movable NDs live adult worms precision $\pm 0.22^{\circ}{\rm C}$. Using this system, determined increase thermogenic responses during chemical stimuli mitochondrial uncouplers. Our technique demonstrates sub-micrometer localization information animals identification pharmacological thermogenesis. The results obtained facilitate development method probe subcellular variation may allow quantification activities expenditures.