Colocalization of protein and microRNA markers reveals unique extracellular vesicle subpopulations for early cancer detection

• DOI: 10.1126/sciadv.adh8689
• Publication Date: 2024-02-28T18:59:54Z
• Authors: Zongbo Li; Kaizhu Guo; Ziting Gao; Junyi Chen; Zuyang Ye; Minghui Cao; Shizhen Emily Wang; Yadong Yin; Wenwan Zhong
• Keywords: Colocalization; Extracellular vesicles; Extracellular Vesicles; Microvesicle

Extracellular vesicles (EVs) play important roles in cell-cell communication but are highly heterogeneous, and each vesicle has dimensions smaller than 200 nm with very limited amounts of cargos encapsulated. The technique NanOstirBar (NOB)-EnabLed Single Particle Analysis (NOBEL-SPA) reported the present work permits rapid inspection single EV high confidence by confocal fluorescence microscopy, thus enables colocalization assessment for selected protein microRNA (miRNA) markers EVs produced various cell lines, or clinical sera samples. subpopulations marked unique miRNA combinations were discovered to be able detect early-stage (stage I II) breast cancer (BC). NOBEL-SPA can adapted analyze other types cargo molecules small submicron biological particles. Study sorting specific heterogeneous under different physiological conditions help discover distinct valuable examination therapeutics development gain better understanding their biogenesis.