Single-shot digital optical fluorescence phase conjugation through forward multiple-scattering samples

• DOI: 10.1126/sciadv.adi1120
• Publication Date: 2024-01-19T18:58:32Z
• Authors: Tengfei Wu; Yixuan Zhang; Baptiste Blochet; Payvand Arjmand; Pascal Berto; Marc Guillon
• Keywords: Phase conjugation; Reference beam

Aberrations and multiple scattering in biological tissues critically distort light beams into highly complex speckle patterns. In this regard, digital optical phase conjugation (DOPC) is a promising technique enabling in-depth focusing. However, DOPC becomes challenging when using fluorescent guide stars for four main reasons: the low photon budget available, large spectral bandwidth of signal, Stokes shift between emission excitation wavelength, absence reference beam preventing holographic measurement. Here, we demonstrate possibility to focus laser through multiple-scattering samples by measuring fields single acquisition step with reference-free, high-resolution wavefront sensor. By taking advantage forward multiply samples, fluorescence achieved at wavelength while broadband field arising from micrometer-sized bead.