Activation of Endogenous Cdc42 Visualized in Living Cells

• DOI: 10.1126/science.1100367
• Publication Date: 2004-09-09T21:17:55Z
• Authors: Perihan Nalbant; Louis Hodgson; Vadim Kraynov; Alexei Toutchkine; Klaus M. Hahn
• Keywords: CDC42

Signaling proteins are tightly regulated spatially and temporally to perform multiple functions. For Cdc42 other guanosine triphosphatases, the subcellular location of activation is a critical determinant cell behavior. However, current approaches limited in their ability examine dynamics activity living cells. We report development biosensor capable visualizing changing endogenous, unlabeled With use dye that reports protein interactions, revealed localized trans-Golgi apparatus, microtubule-dependent at periphery, kinetics precisely coordinated with extension retraction.