Histone Lysine Demethylase JARID1a Activates CLOCK-BMAL1 and Influences the Circadian Clock

• DOI: 10.1126/science.1206022
• Publication Date: 2011-09-29T18:45:44Z
• Authors: Luciano DiTacchio; Hiep D. Le; Christopher Vollmers; Megumi Hatori; Michael Witcher; Julie Secombe; Satchidananda Panda
• Keywords: Demethylase

In animals, circadian oscillators are based on a transcription-translation circuit that revolves around the transcription factors CLOCK and BMAL1. We found JumonjiC (JmjC) ARID domain-containing histone lysine demethylase 1a (JARID1a) formed complex with CLOCK-BMAL1, which was recruited to Per2 promoter. JARID1a increased acetylation by inhibiting deacetylase 1 function enhanced CLOCK-BMAL1 in demethylase-independent manner. Depletion of mammalian cells reduced Per promoter acetylation, dampened expression canonical genes, shortened period rhythms. Drosophila lines Jarid1a homolog, lid, had lowered similarly altered thus has nonredundant role oscillator function.