Delineating Antibody Recognition in Polyclonal Sera from Patterns of HIV-1 Isolate Neutralization

• DOI: 10.1126/science.1233989
• Publication Date: 2013-05-09T18:05:18Z
• Authors: Ivelin S. Georgiev; Nicole A. Doria-Rose; Tongqing Zhou; Young Do Kwon; Ryan P. Staupe; Stephanie Moquin; Gwo-Yu Chuang; Mark K. Louder; Stephen D. Schmidt; Han R. Altae-Tran; Robert T. Bailer; Krisha McKee; Martha Nason; Sijy O’Dell; Gilad Ofek; Marie Pancera; Sanjay Srivatsan; Lawrence Shapiro; Mark Connors; Stephen A. Migueles; Lynn Morris; Yoshiaki Nishimura; Malcolm A. Martin; John R. Mascola; Peter D. Kwong
• Keywords: Immunogen; Polyclonal antibodies

Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities HIV-1-neutralizing antibodies in serum can be elucidated from pattern neutralization against a diverse panel HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing on 34 strains showed similarity fingerprint correlated with epitope. used these fingerprints delineate polyclonal sera 24 HIV-1-infected donors chimeric siman-human immunodeficiency virus-infected macaque. Delineated matched published were further confirmed by two sera. Patterns virus-isolate thus afford detailed epitope-specific neutralizing-antibody responses