Structure of the Yeast Mitochondrial Large Ribosomal Subunit

• DOI: 10.1126/science.1249410
• Publication Date: 2014-03-27T18:25:09Z
• Authors: Alexey Amunts; Alan Brown; Xiao-chen Bai; Jose L. Llácer; Tanweer Hussain; Paul Emsley; Fei Long; Garib Murshudov; Sjors H. W. Scheres; V. Ramakrishnan
• Keywords: Angstrom; Cryo-Electron Microscopy; Eukaryotic Ribosome; Mitochondrial ribosome

Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. We solved the structure of yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution 3.2 angstroms enabled a nearly complete atomic model to be built de novo refined, including 39 proteins, 13 which are unique mitochondria, as well expansion segments RNA. reveals new exit tunnel path architecture, elements E site, putative membrane docking site.