SV2, a Brain Synaptic Vesicle Protein Homologous to Bacterial Transporters

• DOI: 10.1126/science.1519064
• Publication Date: 2006-10-05T23:03:06Z
• Authors: Sandra M. Bajjalieh; Karen Peterson; Rajesh Shinghal; Richard H. Scheller
• Keywords: 0301 basic medicine; Membrane Glycoproteins; Molecular Sequence Data; Brain; Nerve Tissue Proteins; DNA; Transfection; Polymerase Chain Reaction; Rats; Fungal Proteins; 03 medical and health sciences; Bacterial Proteins; Sequence Homology, Nucleic Acid; Animals; Electrophoresis, Polyacrylamide Gel; Amino Acid Sequence; Carrier Proteins

Synaptic vesicle protein 2 (SV2) is a membrane glycoprotein specifically localized to secretory vesicles in neurons and endocrine cells. As first step toward understanding the function of SV2 neural secretion, rat brain complementary DNA (cDNA) that encodes was isolated characterized. Analyses this cDNA predict contains 12 transmembrane domains. The NH -terminal half shows significant amino acid sequence identity family bacterial proteins transport sugars, citrate, drugs. Expression COS cells yielded high level SV2-like immunoreactivity distributed reticular punctate pattern, which suggests localization intracellular membranes. Its vesicles, predicted topology, known transporters suggest synaptic vesicle-specific transporter.