Sulfhydryl Groups and Estradiol-Receptor Interaction

• DOI: 10.1126/science.158.3799.385
• Publication Date: 2006-10-05T11:08:12Z
• Authors: Elwood V. Jensen; Daniel J. Hurst; Eugene R. DeSombre; Peter W. Jungblut
• Keywords: Fraction (chemistry)

The characteristic ability of rat uteri to take up tritiated estradiol in vitro or retain previously incorporated either vivo is destroyed by treating the tissue with various sulfhydryl-blocking reagents. two radioactive estradiol-receptor complexes, observed uterine homogenates supernatant fraction and an extract nuclear fraction, respectively, are disrupted brief exposure organic mercurials cold. Sulfhydryl groups receptor substances apparently play a vital role binding, perhaps indirectly through contribution conformation.