Lutropin-Choriogonadotropin Receptor: An Unusual Member of the G Protein-Coupled Receptor Family

• DOI: 10.1126/science.2502842
• Publication Date: 2006-10-05T21:21:28Z
• Authors: K. C. McFarland; Rolf Sprengel; Heidi S. Phillips; Martin Köhler; Nora Rosemblit; Karoly Nikolics; Deborah L. Segaloff; Peter H. Seeburg
• Keywords: 0303 health sciences; Base Sequence; Molecular Sequence Data; Ovary; Nucleic Acid Hybridization; DNA; Receptors, LH; Rats; 03 medical and health sciences; GTP-Binding Proteins; Sequence Homology, Nucleic Acid; Animals; Female; Tissue Distribution; Amino Acid Sequence; RNA, Messenger; Cloning, Molecular; DNA Probes; Glycoproteins

A complementary DNA (cDNA) for the rat luteal lutropin-choriogonadotropin receptor (LH-CG-R) was isolated with use of a probe generated in polymerase chain reaction oligonucleotide primers based on peptide sequences purified protein. As would be predicted from cDNA sequence, LH-CG-R consists 26-residue signal peptide, 341-residue extracellular domain displaying an internal repeat structure characteristic members leucine-rich glycoprotein (LRG) family, and 333-residue region containing seven transmembrane segments. This membrane-spanning displays sequence similarity all G protein-coupled family. Hence, gene may have evolved by recombination LRG genes. Cells engineered to express bind human choriogonadotropin high affinity show increase cyclic adenosine monophosphate when exposed hormone. revealed RNA blot analysis situ hybridization, 4.4-kilobase cognate messenger is prominently localized ovary.