Protein-RNA Interactions in an Icosahedral Virus at 3.0 Å Resolution

• DOI: 10.1126/science.2749253
• Publication Date: 2006-10-05T21:21:28Z
• Authors: Zhongguo Chen; Cynthia Stauffacher; Yunge Li; Tim Schmidt; Wu Bomu; Greg Kamer; Michael Shanks; George Lomonossoff; John E. Johnson
• Keywords: Protein quaternary structure; Picornaviridae; RNA virus

Nearly 20 percent of the packaged RNA in bean-pod mottle virus (BPMV) binds to capsid interior a symmetric fashion and is clearly visible electron density map. The displaying icosahedral symmetry single-stranded with well-defined polarity stereochemical properties. Interactions protein are dominated by nonbonding forces few specific contacts. tertiary quaternary structures BPMV proteins similar those observed animal picornaviruses, supporting close relation between plant comoviruses picornaviruses established previous biological studies.