Role of Dynamin in the Formation of Transport Vesicles from the Trans-Golgi Network

• DOI: 10.1126/science.279.5350.573
• Publication Date: 2002-07-27T09:37:46Z
• Authors: Steven M. Jones; Kathryn E. Howell; John R. Henley; Hong Cao; Mark A. McNiven
• Keywords: Dynamins; Organelles; 0303 health sciences; Recombinant Fusion Proteins; Cell Membrane; Green Fluorescent Proteins; Coated Vesicles; Golgi Apparatus; Biological Transport; Clathrin; GTP Phosphohydrolases; Rats; Luminescent Proteins; 03 medical and health sciences; Adenosine Triphosphate; Cytosol; Liver; Microscopy, Fluorescence; Guanosine 5'-O-(3-Thiotriphosphate); Animals; Cells, Cultured

Dynamin guanosine triphosphatases support the scission of clathrin-coated vesicles from plasmalemma during endocytosis. By fluorescence microscopy cultured rat hepatocytes, a green fluorescent protein–dynamin II fusion protein localized with at Golgi complex. A cell-free assay was utilized to demonstrate role dynamin in vesicle formation trans-Golgi. Addition peptide-specific anti-dynamin antibodies mixture inhibited both constitutive exocytic and formation. Immunodepletion proteins also formation, budding efficiency restored upon readdition purified dynamin. These data suggest that participates distinct transport trans-Golgi network.