A Functional Assay for Centromere-Associated Sister Chromatid Cohesion

• DOI: 10.1126/science.285.5425.254
• Publication Date: 2002-07-27T09:42:20Z
• Authors: Paul C. Megee; Douglas Koshland
• Keywords: Recombination, Genetic; 0301 basic medicine; Centromere; Green Fluorescent Proteins; G1 Phase; Mitosis; Saccharomyces cerevisiae; Chromatids; Luminescent Proteins; 03 medical and health sciences; Chromosomes, Fungal; DNA, Fungal; Kinetochores; Conserved Sequence; In Situ Hybridization, Fluorescence

Cohesion of sister chromatids occurs along the entire length chromosomes, including centromere where it plays essential roles in chromosome segregation. Here, minichromosomes budding yeast Saccharomyces cerevisiae are exploited to generate a functional assay for DNA sequences involved cohesion. The centromeric element CDEIII was found be necessary but not sufficient This shown previously required assembly kinetochore, centromere-associated protein complex that attaches chromosomes spindle. These observations establish link between centromere-proximal cohesion and kinetochore assembly.