Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis

• DOI: 10.1126/science.285.5429.901
• Publication Date: 2002-07-27T09:37:20Z
• Authors: Elizabeth A. Winz...; Daniel D. Shoemaker; Anna Astromoff; Hong Liang; Keith Anderson; Bruno Andre; Rhonda Bangham; Rocio Benito; Jef D. Boeke; Howard Bussey; Angela M. Chu; Carla Connelly; Karen Davis; Fred Dietrich; Sally Whelen Dow; Mohamed El Bakkoury; Françoise Foury; Stephen H. Friend; Erik Gentalen; Guri Giaever; Johannes H. Hegemann; Ted Jones; Michael Laub; Hong Liao; Nicole Liebundguth; David J. Lockhart; Anca Lucau-Danila; Marc Lussier; Nasiha M'Rabet; Patrice Menard; Michael Mittmann; Chai Pai; Corinne Rebischung; Jose L. Revuelta; Linda Riles; Christopher J. Ro...; Petra Ross-MacDonald; Bart Scherens; Michael Snyder; Sharon Sookhai-Ma...; Reginald K. Storms; Steeve Véronneau; Marleen Voet; Guido Volckaert; Teresa R. Ward; Robert Wysocki; Grace S. Yen; Kexin Yu; Katja Zimmermann; Peter Philippsen; Mark Johnston; Ronald W. Davis
• Keywords: ORFS; Genome size

The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches required to systematically determine their function. A total 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with precise deletion one 2026 ORFs (more than one-third the genome). Of deleted ORFs, 17 percent essential for viability rich medium. phenotypes more 500 assayed parallel. strains, 40 showed quantitative growth defects either or minimal