Role of the Protein Chaperone YDJ1 in Establishing Hsp90-Mediated Signal Transduction Pathways

• DOI: 10.1126/science.7761857
• Publication Date: 2006-10-27T18:19:42Z
• Authors: Yoko Kimura; Ichiro Yahara; Susan Lindquist
• Keywords: CDC37; Chaperone (clinical)

The substrate-specific protein chaperone Hsp90 (heat shock 90) from Saccharomyces cerevisiae functions in diverse signal transduction pathways. A mutation YDJ1 , a member of the DnaJ family, was recovered synthetic-lethal screen with mutants. In an otherwise wild-type background, ydj1 exerted strong and specific effects on three substrates, derepressing two (the estrogen glucocorticoid receptors) reducing function third tyrosine kinase p60 v- src ). Analysis one these receptor, indicated that Ydj1 exerts its through physical interaction substrates.