The Effect of Posttranslational Modifications on the Interaction of Ras2 with Adenylyl Cyclase

• DOI: 10.1126/science.8430318
• Publication Date: 2006-10-05T23:05:09Z
• Authors: Yuichi Kuroda; Noboru Suzuki; Tohru Kataoka
• Keywords: Palmitoylation; ADCY10; ADCY6

Ras proteins undergo a series of posttranslational modifications that are critical for their cellular function. These necessary to anchor the membrane. Yeast Ras2 were purified with various degrees modification and examined ability activate effector, adenylyl cyclase. The farnesylated intermediate form had more than 100 times higher affinity cyclase unprocessed form. subsequent palmitoylation reaction little effect. In contrast, was required efficient membrane localization protein. results indicate importance farnesylation in interaction its effector.