Transcriptome-wide distribution and function of RNA hydroxymethylcytosine

• DOI: 10.1126/science.aac5253
• Publication Date: 2016-01-15T03:23:17Z
• Authors: Benjamin Delatte; Fei Wang; Long Vo Ngoc; Evelyne Collignon; Elise Bonvin; Rachel Deplus; Emilie Calonne; Bouchra Hassabi; Pascale Putmans; Stephan Awe; Collin Wetzel; Judith Kreher; Romuald Soin; Catherine Creppe; Patrick A. Limbach; Cyril Gueydan; Véronique Kruys; Alexander Brehm; Svetlana Minakhina; Matthieu Defrance; Ruth Steward; François Fuks
• Keywords: 5-Hydroxymethylcytosine

Hydroxymethylcytosine, well described in DNA, occurs also RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing transcripts of many genes, notably coding sequences, identify consensus sites for hydroxymethylation. found RNA can favor mRNA translation. hydroxymethylated are to be most abundant Drosophila brain, Tet-deficient fruitflies suffer impaired brain development, accompanied decreased This study highlights distribution, localization, function cytosine identifies central roles this modification