Comprehensive single-cell transcriptional profiling of a multicellular organism

• DOI: 10.1126/science.aam8940
• Publication Date: 2017-08-17T18:00:28Z
• Authors: Junyue Cao; Jonathan S. Packer; Vijay Ramani; Darren A. Cusanovich; Chau Huynh; Riza Daza; Xiaojie Qiu; Choli Lee; Scott N. Furlan; Frank J. Steemers; Andrew Adey; Robert H. Waterston; Cole Trapnell; Jay Shendure
• Keywords: Multicellular organism; Single-Cell Analysis; Single cell sequencing; Model Organism; Cell type; Egg cell

Sequencing each cell of the nematode Single-cell sequencing is challenging owing to limited biological material available in an individual and high cost across multiple cells. Cao et al. developed a two-step combinatorial barcoding method profile both single-cell single-nucleus transcriptomes without requiring physical isolation cell. The authors profiled almost 50,000 single cells from Caenorhabditis elegans larval stage were able identify recover information different, even rare, types. Science , this issue p. 661