Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types

• DOI: 10.1126/science.abm8797
• Publication Date: 2022-11-03T17:58:37Z
• Authors: Madhuvanthi Kannan; Ganesh Vasan; Simon Haziza; Cheng Huang; Radosław Chrapkie...; Junjie Luo; Jessica A. Cardin; Mark J. Schnitzer; Vincent A. Pieribone
• Keywords: Polarity (international relations)

Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current lack requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green red sensors, Ace-mNeon2 VARNAM2, their reverse response-polarity variants pAce pAceR. Our enable 0.4- 1-kilohertz recordings from >50 spiking neurons per field of view awake mice ~30-minute continuous imaging flies. Using dual-polarity multiplexed imaging, we uncovered brain state-dependent antagonism neocortical somatostatin-expressing (SST+) vasoactive intestinal peptide-expressing (VIP+) interneurons contributions hippocampal potentials ensembles with distinct axonal projections. By combining three mutually compatible indicators, performed simultaneous triple-population These approaches will empower investigations dynamic interplay neuronal subclasses at single-spike resolution.