Translatome and transcriptome co-profiling reveals a role of TPRXs in human zygotic genome activation

• DOI: 10.1126/science.abo7923
• Publication Date: 2022-09-08T18:00:07Z
• Authors: Zhuoning Zou; Chuanxin Zhang; Qiuyan Wang; Zhenzhen Hou; Zhuqing Xiong; Feng Kong; Qiujun Wang; Jinzhu Song; Boyang Liu; Bofeng Liu; Lijuan Wang; Fangnong Lai; Qiang Fan; Wenrong Tao; Shuai Zhao; Xiaonan Ma; Miao Li; Keliang Wu; Han Zhao; Zi-Jiang Chen; Wei Xie
• Keywords: Reprogramming; Ribosome profiling

Translational regulation plays a critical role during the oocyte-to-embryo transition (OET) and zygotic genome activation (ZGA). Here, we integrated ultra-low-input ribosome profiling (Ribo-lite) with messenger RNA sequencing to co-profile translatome transcriptome in human oocytes early embryos. Comparison mouse counterparts identified widespread differentially translated gene functioning epigenetic reprogramming, transposon defense, small biogenesis, part driven by species-specific regulatory elements 3' untranslated regions. Moreover, PRD-like homeobox transcription factors, including TPRXL, TPRX1, TPRX2, are highly around ZGA. TPRX1/2/L knockdown leads defective ZGA preimplantation development. Ectopically expressed TPRXs bind activate key genes embryonic stem cells. These data reveal conservation divergence of translation landscapes OET identify regulators