Noninvasive Identification and Monitoring of Cancer Mutations by Targeted Deep Sequencing of Plasma DNA

• DOI: 10.1126/scitranslmed.3003726
• Publication Date: 2012-05-30T18:30:27Z
• Authors: Tim Forshew; Muhammed Murtaza; Christine Parkinson; Davina Gale; Dana W. Y. Tsui; Fiona Kaper; Sarah-Jane Dawson; Anna M. Piskorz; Mercedes Jimenez-...; David Bentley; James Hadfield; Andrew P. May; Carlos Caldas; James D. Brenton; Nitzan Rosenfeld
• Keywords: Amplicon; Identification; Circulating tumor DNA

Sizable genomic regions were screened and low-frequency mutations identified in circulating DNA of cancer patients using tagged-amplicon deep sequencing (TAm-Seq).