Identification and Characterization of Linezolid-Resistantcfr-Positive Staphylococcus aureus USA300 Isolates from a New York City Medical Center

• DOI: 10.1128/aac.03380-14
• Publication Date: 2014-08-19T05:54:44Z
• Authors: Jeffrey B. Locke; Douglas E. Zuill; Caitlyn R. Scharn; Jennifer Deane; Daniel F. Sahm; Richard V. Goering; Stephen G. Jenkins; Karen J. Shaw
• Keywords: Methicillin-Resistant Staphylococcus aureus; Thiamphenicol; 0303 health sciences; Clindamycin; Molecular Sequence Data; Gene Transfer Techniques; Linezolid; New York; Microbial Sensitivity Tests; Staphylococcal Infections; Organophosphates; Anti-Bacterial Agents; 3. Good health; 03 medical and health sciences; Bacterial Proteins; Drug Resistance, Multiple, Bacterial; Acetamides; Humans; Diterpenes; Oxazoles; Oxazolidinones; Plasmids

ABSTRACTThecfrgene was identified in three linezolid-resistant USA300 methicillin-resistantStaphylococcus aureus(MRSA) isolates collected over a 3-day period at a New York City medical center in 2011 as part of a routine surveillance program. Each isolate possessed a plasmid containing a pSCFS3-likecfrgene environment. Transformation of thecfr-bearing plasmids into theS. aureusATCC 29213 background recapitulated the expected Cfr antibiogram, including resistance to linezolid, tiamulin, clindamycin, and florfenicol and susceptibility to tedizolid.