Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations

• DOI: 10.1128/aac.37.3.457
• Publication Date: 2012-06-28T00:05:50Z
• Authors: Y Wang; W M Huang; D E Taylor
• Keywords: DNA, Bacterial; 0301 basic medicine; 4-Quinolones; Base Sequence; Molecular Sequence Data; Chromosome Mapping; Drug Resistance, Microbial; Chromosomes, Bacterial; 3. Good health; Campylobacter jejuni; 03 medical and health sciences; DNA Topoisomerases, Type II; Anti-Infective Agents; Genes, Bacterial; Mutation; Topoisomerase II Inhibitors; Amino Acid Sequence; Cloning, Molecular

The gyrA gene of Campylobacter jejuni UA580, which encodes the A subunit DNA gyrase, was cloned and its nucleotide sequence determined. An open reading frame 2,589 nucleotides identified, could code for a polypeptide 863 amino acids with M(r) 97 kDa. Both putative acid show ca. 50% identity those other genes from gram-positive gram-negative bacteria. locations on genome maps both C. UA580 coli UA417 were Six nalidixic acid-resistant isolates shown to carry mutations in gyrA. Three clinical had Thr-86-to-Ile substitutions. laboratory mutants substitutions Thr-86 Ile, Asp-90 Ala, Ala-70 Thr, respectively. mutation at Thr-86, is homologous Ser-83 Escherichia coli, associated high-level resistance ciprofloxacin jejuni.