In Vitro and In Vivo Activities of Levofloxacin against Biofilm-Producing Pseudomonas aeruginosa

• DOI: 10.1128/aac.42.7.1641
• Publication Date: 2018-10-09T00:33:03Z
• Authors: Hiroko Ishida; Yoshihisa Ishida; Yuichi Kurosaka; Tsuyoshi Otani; Kenichi Sato; Hiroyuki Kobayashi
• Keywords: 0301 basic medicine; Ofloxacin; Alginates; Hexuronic Acids; Guinea Pigs; Levofloxacin; Microbial Sensitivity Tests; Permeability; 3. Good health; 03 medical and health sciences; Anti-Infective Agents; Glucuronic Acid; Biofilms; Pseudomonas aeruginosa; Pneumonia, Bacterial; Animals; Female; Pseudomonas Infections; Polytetrafluoroethylene

Interactions between biofilm cells of Pseudomonas aeruginosa and levofloxacin were studied. P. incubated for 6 days with Teflon sheets formed a on its surface. Against the bacteria, at an MIC determined by standard method strain was highly bactericidal whereas gentamicin, ceftazidime, ciprofloxacin showed no significant killing activity. Levofloxacin, ciprofloxacin, but not exhibited activity against nongrowing in phosphate buffer. In addition, levofloxacin, ability to penetrate agar containing alginate. These findings may explain efficacy ineffectiveness gentamicin ceftazidime bacteria; however, cause still remains be determined. experimental pneumonia guinea pigs, which mode growth observed lung, only substantial therapeutic efficacy. suggest role therapy bacterium-associated infectious diseases.