Genetic Analysis of Azole Resistance in the Darlington Strain of Candida albicans

• DOI: 10.1128/aac.44.11.2985-2990.2000
• Publication Date: 2002-07-27T10:06:42Z
• Authors: Hiroshi Kakeya; Yoshitsugu Miyazaki; Haruko Miyazaki; Katherine Nyswaner; Brian Grimberg; John E. Bennett
• Keywords: Azole; Strain (injury)

High-level azole resistance in the Darlington strain of Candida albicans was investigated by gene replacement C. and expression Saccharomyces cerevisiae. We sequenced ERG11 gene, which encodes sterol C(14)alpha-demethylase, from our copy strain. Both alleles contained histidine for tyrosine substitution at position 132 (Y132H) reported others, but we also found a threonine-for-isoleucine (I471T) not previously ERG11. The encoded I471T change amino acids conferred when overexpressed alone increased added to Y132H acid sequence an S. cerevisiae system. Replacement one azole-susceptible with single resulted integrated modest increase resistance. profound is result multiple mutations.