Commensal Escherichia coli Aggravates Acute Necrotizing Pancreatitis through Targeting of Intestinal Epithelial Cells

• DOI: 10.1128/aem.00059-19
• Publication Date: 2019-04-12T10:07:57Z
• Authors: Junyuan Zheng; Lihong Lou; Junjie Fan; Chunlan Huang; Qixiang Mei; Jianghong Wu; Yuecheng Guo; Yingying Lu; Xingpeng Wang; Yue Zeng
• Keywords: Escherichia coli infection; Barrier function

An increase of Escherichia-Shigella was previously reported in acute necrotizing pancreatitis (ANP). We investigated whether Escherichia coli MG1655, an commensal organism, increased intestinal injury and aggravated ANP rats. induced by retrograde injection 3.5% sodium taurocholate into the biliopancreatic duct. Using gut microbiota-depleted rats, we demonstrated that microbiota involved pancreatic barrier dysfunction ANP. 16S rRNA gene sequencing quantitative PCR, found dysbiosis a significant E. MG1655 Afterward, administration gavage to rats with performed. observed after induction, MG1655-monocolonized presented more severe pancreas function than Furthermore, Toll-like receptor 4 (TLR4)/MyD88/p38 mitogen-activated protein (MAPK) endoplasmic reticulum stress (ERS) activation epithelial cells were also significantly In vitro, rat ileal cell line IEC-18 displayed tumor necrosis factor alpha-induced inflammation loss tight-junction proteins coculture as well TLR4, MyD88, Bip upregulation. conclusion, our study shows increases TLR4/MyD88/p38 MAPK ERS signaling-induced aggravates Our describes harmful potential ANP.IMPORTANCE This ANP, which has not been previous studies. work provides new insights bacterium-ANP cross talk, suggesting nonpathogenic commensals could exhibit adverse effects context diseases.