Expression of Reductive Dehalogenase Genes in Dehalococcoides ethenogenes Strain 195 Growing on Tetrachloroethene, Trichloroethene, or 2,3-Dichlorophenol

• DOI: 10.1128/aem.00215-07
• Publication Date: 2007-05-19T14:57:56Z
• Authors: Jennifer M. Fung; Robert M. Morris; Lorenz Adrian; Stephen H. Zinder
• Keywords: Dehalogenase; Dehalococcoides; rpoB; Reductive Dechlorination; Enrichment culture; Electron acceptor

Reductive dehalogenase (RD) gene transcript levels in Dehalococcoides ethenogenes strain 195 were investigated using reverse transcriptase quantitative PCR during growth and reductive dechlorination of tetrachloroethene (PCE), trichloroethene (TCE), or 2,3-dichlorophenol (2,3-DCP). Cells grown with PCE TCE had high (greater than that for rpoB) tceA, which encodes the RD, pceA, DET0162, contains a predicted stop codon is considered nonfunctional. In cells 2,3-DCP, tceA mRNA was less 1% rpoB, indicating its transcription regulated. pceA DET0162 only RD genes 2,3-DCP. Proteomic analysis PCE-grown detected both PceA TceA peptide coverage but not 2,3-DCP-grown TceA, any other potential RD. 2,3-DCP tested ability to dechlorinate PCE, TCE, H2 as electron donor. unable dechlorinated without lag, lag. These results show do produce transcribed translation product detectable are consistent PceA's being bifunctional, also serving Chlorophenols naturally occur soils good candidates original substrates PceA.