Biphenyl-Metabolizing Bacteria in the Rhizosphere of Horseradish and Bulk Soil Contaminated by Polychlorinated Biphenyls as Revealed by Stable Isotope Probing

• DOI: 10.1128/aem.00466-09
• Publication Date: 2009-08-22T01:50:29Z
• Authors: Ondrej Uhlik; Katerina Jecna; Martina Mackova; Cestmir Vlcek; Miluse Hroudova; Katerina Demnerova; Vaclav Paces; Tomas Macek
• Keywords: Stable-isotope probing; Achromobacter; Achromobacter xylosoxidans; Biphenyl; Bulk soil; Alcaligenes; Soil microbiology; Betaproteobacteria

DNA-based stable isotope probing in combination with terminal restriction fragment length polymorphism was used order to identify members of the microbial community that metabolize biphenyl rhizosphere horseradish (Armoracia rusticana) cultivated soil contaminated polychlorinated biphenyls (PCBs) compared initial, uncultivated bulk soil. On basis early and recurrent detection their 16S rRNA genes clone libraries constructed from [(13)C]DNA, Hydrogenophaga spp. appeared dominate catabolism soil, whereas Paenibacillus were predominant biphenyl-utilizing bacteria initial Other found derive carbon this nutrient-amended microcosm-based study belonged mostly class Betaproteobacteria identified as Achromobacter spp., Variovorax Methylovorus or Methylophilus Some unclassified at genus level also detected, these may be undescribed genera. The deduced amino acid sequences dioxygenase alpha subunits (BphA) incorporated [(13)C]into DNA 3-day incubations soils [(13)C]biphenyl are almost identical Pseudomonas alcaligenes B-357. This suggests spectrum PCB congeners can degraded by enzymes similar strain These results demonstrate altering environment result participation different metabolism biphenyl.