13,16-Dimethyl Octacosanedioic Acid ( iso -Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3

• DOI: 10.1128/aem.00466-11
• Publication Date: 2011-04-23T03:29:25Z
• Authors: Jaap S. Sinninghe...; W. Irene C. Rijpstra; Ellen C. Hopmans; Johan W. H. Weijers; Bärbel U. Foesel; Jörg Overmann; Svetlana N. Dedysh
• Keywords: Acidobacteria

ABSTRACT The distribution of membrane lipids 17 different strains representing 13 species subdivisions 1 and 3 the phylum Acidobacteria , a highly diverse Bacteria were examined by hydrolysis gas chromatography-mass spectrometry (MS) high-performance liquid chromatography-MS intact polar lipids. Upon both acid base hydrolyses total cell material, uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic ( iso -diabolic acid) was released in substantial amounts (22 to 43% fatty acids) from all acidobacteria studied. This has previously been encountered only thermophilic Thermoanaerobacter but bears structural resemblance alkyl chains bacterial glycerol dialkyl tetraethers (GDGTs) that occur ubiquitously peat soil are suspected be produced acidobacteria. As reported previously, most also contained -C 15 C 16:1ω7C as major acids presence unnoticed previous studies, probably because complex this moiety not extractable cells; it could hydrolysis. Direct analysis Bligh-Dyer extract three acidobacterial strains, indeed, did reveal any containing acid. In ether-bound detected after cells, including one branched GDGT acid-derived chains. Since soils is much more complex, GDGTs likely originate other (acido)bacteria capable biosynthesizing these components.