Rumen Microbiome Composition Determined Using Two Nutritional Models of Subacute Ruminal Acidosis

• DOI: 10.1128/aem.00739-09
• Publication Date: 2009-09-26T01:51:42Z
• Authors: Ehsan Khafipour; Shucong Li; Jan C. Plaizier; Denis O. Krause
• Keywords: Prevotella; Streptococcus bovis

Subacute ruminal acidosis (SARA) is a metabolic disease in dairy cattle that occurs during early and mid-lactation has traditionally been characterized by low rumen pH, but lactic acid does not accumulate as acute acidosis. It hypothesized factors such increased gut permeability, bacterial lipopolysaccharides, inflammatory responses may have role the etiology of SARA. However, little known about nature microbiome In this study, we analyzed 64 samples taken from eight lactating Holstein using terminal restriction fragment length polymorphisms (TRFLP) 16S rRNA genes real-time PCR. We used two published experiments which SARA had induced with either grain or alfalfa pellets. The results TRFLP analysis indicated most predominant shift was decline gram-negative Bacteroidetes organisms. proportion organisms greater pellet-induced than mild severe grain-induced (35.4% versus 26.0% 16.6%, respectively). This also evident PCR data for Prevotella albensis, brevis, ruminicola, are members Bacteroidetes. dominated Streptococcus bovis Escherichia coli, whereas Megasphaera elsdenii P. albensis. Using discriminant analysis, severity degree inflammation were highly correlated abundance E. coli lipopolysaccharide rumen. thus suspect be contributing factor onset.