Characterization of a Family GH5 Xylanase with Activity on Neutral Oligosaccharides and Evaluation as a Pulp Bleaching Aid

• DOI: 10.1128/aem.00871-10
• Publication Date: 2010-07-24T02:00:37Z
• Authors: Óscar Gallardo; María Fernández...; Cristina Valls; Susana Valeria Va...; M. Blanca Roncero; Teresa Vidal; Pilar Díaz; F. I. Javier Pastor
• Keywords: 0301 basic medicine; Eucalyptus; Base Sequence; Molecular Sequence Data; Temperature; Oligosaccharides; Bacillus; Sequence Analysis, DNA; Hydrogen-Ion Concentration; Chromatography, Ion Exchange; Lignin; Bleaching Agents; 03 medical and health sciences; Xylosidases; Chromatography, Gel; Amino Acid Sequence; Chromatography, Thin Layer; Cloning, Molecular; Chromatography, Liquid

A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference other GH5 xylanases characterized date that show requirement for methyl-glucuronic acid side chains catalysis. enzyme evaluated Eucalyptus kraft pulp, its effectiveness as bleaching aid.