Cryptosporidium Genotypes in Wildlife from a New York Watershed

• DOI: 10.1128/aem.01034-07
• Publication Date: 2007-08-25T01:08:40Z
• Authors: Yaoyu Feng; Kerri A. Alderisio; Wenli Yang; Lisa A. Blancero; William G. Kuhne; Christopher A. Na...; Michael Reid; Lihua Xiao
• Keywords: 0301 basic medicine; Base Sequence; Genotype; Molecular Sequence Data; Cryptosporidiosis; Cryptosporidium; Animals, Wild; Fresh Water; Sequence Analysis, DNA; 6. Clean water; 3. Good health; 03 medical and health sciences; Species Specificity; RNA, Ribosomal; Host-Pathogen Interactions; Animals; Humans; New York City; Phylogeny

To identify the animal sources for Cryptosporidium contamination, we genotyped spp. in wildlife from watershed of New York City drinking water supply, using a small-subunit rRNA gene-based PCR-restriction fragment length polymorphism analysis and DNA sequencing. A total 541 specimens 38 species were analyzed. One hundred eleven (20.5%) PCR positive. Altogether, 21 genotypes found samples, 11 which previously storm runoff watershed, six these unknown origin. Four new found, hosts four expanded. With exception cervine genotype, most limited number have no major public health significance.