Ether- and Ester-Bound iso -Diabolic Acid and Other Lipids in Members of Acidobacteria Subdivision 4

• DOI: 10.1128/aem.01066-14
• Publication Date: 2014-06-16T12:42:14Z
• Authors: Jaap S. Sinninghe...; W. Irene C. Rijpstra; Ellen C. Hopmans; Bärbel U. Foesel; Pia K. Wüst; Jörg Overmann; Marcus Tank; Donald A. Bryant; Peter F. Dunfield; Karen Houghton; Matthew B. Stott
• Keywords: Acidobacteria

ABSTRACT Recently, iso -diabolic acid (13,16-dimethyl octacosanedioic acid) has been identified as a major membrane-spanning lipid of subdivisions 1 and 3 the Acidobacteria , highly diverse phylum within Bacteria . This finding pointed to potential source for bacterial glycerol dialkyl tetraethers that occur ubiquitously in peat, soil, lakes, hot springs. Here, we examined composition seven phylogenetically divergent strains subdivision 4 group is commonly encountered soil. Acid hydrolysis total cell material released derivatives substantial quantities (11 48% all fatty acids). In contrast 6 out 7 species (excepting “ Candidatus Chloracidobacterium thermophilum”) contained ether bound larger fractional abundance than itself. agreement with analysis intact polar lipids (IPLs) by high-performance liquid chromatography-mass spectrometry (HPLC-MS), which showed dominance mixed ether-ester glycerides. -Diabolic acid-containing IPLs were not identified, because these are Bligh-Dyer extraction, observed before when studying compositions The presence bonds membrane does seem be an adaptation temperature, five mesophilic isolates amount thermophile Ca thermophilum.” Furthermore, experiments Pyrinomonas methylaliphatogenes did reveal influence growth temperature over 50 69°C range.