How Diverse Is the GenusWolbachia? Multiple-Gene Sequencing Reveals a Putatively NewWolbachiaSupergroup Recovered from Spider Mites (Acari: Tetranychidae)

• DOI: 10.1128/aem.01109-08
• Publication Date: 2008-12-20T02:11:10Z
• Authors: Vera I. D. Ros; Vicki M. Fleming; Edward J. Feil; Johannes A. J. Br...
• Keywords: DNA, Bacterial; bacterium wolbachia; cytoplasmic incompatibility; genome sequence; Molecular Sequence Data; 590; arthropods; DNA, Ribosomal; 576; Evolution, Molecular; 03 medical and health sciences; pipientis; Bacterial Proteins; RNA, Ribosomal, 16S; Sequence Homology, Nucleic Acid; Animals; Cluster Analysis; Phylogeny; dna amplification; filarial nematodes; f-supergroup; Recombination, Genetic; 0303 health sciences; Polymorphism, Genetic; phylogenetic analysis; reproductive incompatibility; Genes, rRNA; Sequence Analysis, DNA; RNA, Bacterial; Tetranychidae; Wolbachia

ABSTRACT At least 20% of all arthropods and some nematode species are infected with intracellular bacteria the genus Wolbachia . This highly diverse has been subdivided into eight “supergroups” (A to H) on basis nucleotide sequence data. Here, we report discovery a new supergroup recovered from spider mite Bryobia V (Acari: Tetranychidae), based sequences three protein-coding genes ( ftsZ , gltA groEL ) 16S rRNA gene. Other tetranychid mites possess B strains. The another expands known diversity emphasizes high variability genus. Our data also clarify existing structure highlight use multiple gene for robust phylogenetic analysis. In addition previous reports recombination between arthropod-infecting supergroups A B, provide evidence nematode-infecting C D. Robust delineation is essential understanding origin spread this common reproductive parasite unraveling mechanisms host adaptation manipulation across wide range hosts.