Temperature-responsive regulation of the polycyclic aromatic hydrocarbon-degrading mesophilic bacterium Novosphingobium pentaromativorans US6-1 with a temperature adaptation system

• DOI: 10.1128/aem.01484-24
• Publication Date: 2024-12-12T14:00:22Z
• Authors: Zhuangzhuang Liu; Xinran Liu; Haiyan Huang; Feifei Cao; Qiu Meng; Tingheng Zhu; Jianhua Yin; Xiaofei Song; Zhiliang Yu
• Keywords: Mesophile; Polycyclic aromatic hydrocarbon

Survivability and tolerance of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria in harsh environments, especially under varying temperatures, are a bottleneck for the effective application situ bioremediation. In this study, temperature adaptation system (TAS) was constructed by combining customized thermotolerant with cold-resistant to realize temperature-responsive regulation PAH-degrading mesophilic bacterium Novosphingobium pentaromativorans US6-1. The innovative dual-pronged TAS strategy enabled chassis strain effectively tackle conditions ensuring robust biological activities across broadened spectrum exhibiting potential high-efficiency PAH degradation N. US6-1 Furthermore, achieved using circuit is likely promising creating intelligent microbial cell factories avoiding precise maintenance, making it highly useful industrial applications.IMPORTANCEEnvironmental among extremely important factors that determine bioactivities pollutant-degrading microorganisms Effectively maintaining survivability temperatures remains challenge pollutant This first time, developed US6-1, thus diminishing need control