Inhibition Studies with 2-Bromoethanesulfonate Reveal a Novel Syntrophic Relationship in Anaerobic Oleate Degradation

• DOI: 10.1128/aem.01733-18
• Publication Date: 2018-10-26T13:52:04Z
• Authors: A. F. Salvador; A. J. Cavaleiro; A. M. S. Paulo; S. A. Silva; A. P. Guedes; M. A. Pereira; A. J. M. Stams; D. Z. Sousa; M. M. Alves
• Keywords: 2-bromoethanesulfonate (BrES); Clostridiales; 0303 health sciences; Science & Technology; Engenharia e Tecnologia::Biotecnologia Industrial; Methanobacterium; Isethionate; Desulfonation; desulfonation; 6. Clean water; Syntrophomonas; oleate; 03 medical and health sciences; Alkanesulfonic Acids; Oleate; syntrophy; Environmental Microbiology; Desulfovibrio; Methanomicrobiaceae; Anaerobiosis; Syntroph; isethionate; Oleic Acid

In anaerobic treatment of complex wastewater containing fat, oils, and grease, high long-chain fatty acid (LCFA) concentrations may inhibit microbial communities, particularly those of methanogens. Here, we investigated if anaerobic degradation of LCFAs can proceed when methanogens are inhibited and in the absence of typical external electron acceptors, such as nitrate, iron, or sulfate. Inhibition studies were performed with the methanogenic inhibitor 2-bromoethanesulfonate (BrES). We noticed that, after autoclaving, BrES underwent partial hydrolysis and turned out to be a mixture of two sulfonates (BrES and isethionate). We found out that LCFA conversion proceeded faster in the assays where methanogenesis was inhibited, and that it was dependent on the utilization of isethionate. In this study, we report LCFA degradation coupled to desulfonation. Our results also showed that BrES can be utilized by anaerobic bacteria.