Molecular Evidence for Multiple Infections as Revealed by Typing of Asaia Bacterial Symbionts of Four Mosquito Species

• DOI: 10.1128/aem.01747-10
• Publication Date: 2010-09-18T01:12:08Z
• Authors: Bessem Chouaia; Paolo Rossi; Matteo Montagna; Irene Ricci; Elena Crotti; Claudia Damiani; Sara Epis; Ingrid Faye; N'Fale Sagnon; Alberto Alma; Guido Favia; Daniele Daffonchio; Claudio Bandi
• Keywords: Anopheles stephensi; Aedes albopictus; Anopheles gambiae; Multilocus sequence typing; Phylotype

ABSTRACT The recent increased detection of acetic acid bacteria (AAB) the genus Asaia as symbionts mosquitoes, such Anopheles spp. and Aedes spp., prompted us to investigate diversity these their relationships in different mosquito species populations. Following cultivation-dependent -independent techniques, we investigated microbiota associated with four species, stephensi , gambiae aegypti albopictus which are important vectors human and/or animal pathogens. Denaturing gradient gel electrophoresis (DGGE) analysis based on 16S rRNA gene revealed presence several bacterial taxa, among sequences were dominant most samples. A collection 281 isolates cell-free media was established from individuals belonging species. typed by internal transcribed spacer (ITS)-PCR, tRNA-PCR, BOX-PCR, randomly amplified polymorphic DNA (RAPD)-PCR, revealing that strains present populations, even single individuals.