The Core Gut Microbiome of the American Cockroach, Periplaneta americana, Is Stable and Resilient to Dietary Shifts

• DOI: 10.1128/aem.01837-16
• Publication Date: 2016-09-03T02:38:28Z
• Authors: Kara A. Tinker; Elizabeth A. Ottesen
• Keywords: 2. Zero hunger; 0303 health sciences; Bacteria; Bacteroidetes; Firmicutes; Genetic Variation; Biodiversity; Sequence Analysis, DNA; 15. Life on land; Diet; Gastrointestinal Microbiome; Feces; 03 medical and health sciences; RNA, Ribosomal, 16S; Proteobacteria; Animals; Humans; Periplaneta; Digestion; Phylogeny

ABSTRACTThe omnivorous cockroachPeriplaneta americanahosts a diverse hindgut microbiota encompassing hundreds of microbial species. In this study, we used 16S rRNA gene sequencing to examine the effect of diet on the composition of theP. americanahindgut microbial community. Results show that the hindgut microbiota ofP. americanaexhibit a highly stable core microbial community with low variance in compositions between individuals and minimal community change in response to dietary shifts. This core hindgut microbiome is shared between laboratory-hosted and wild-caught individuals, although wild-caught specimens exhibited a higher diversity of low-abundance microbes that were lost following extended cultivation under laboratory conditions. This taxonomic stability strongly contrasts with observations of the gut microbiota of mammals, which have been shown to be highly responsive to dietary change. A comparison ofP. americanahindgut samples with human fecal samples indicated that the cockroach hindgut community exhibited higher alpha diversity but a substantially lower beta diversity than the human gut microbiome. This suggests that cockroaches have evolved unique mechanisms for establishing and maintaining a diverse and stable core microbiome.IMPORTANCEThe gut microbiome plays an important role in the overall health of its host. A healthy gut microbiota typically assists with defense against pathogens and the digestion and absorption of nutrients from food, while dysbiosis of the gut microbiota has been associated with reduced health. In this study, we examined the composition and stability of the gut microbiota from the omnivorous cockroachPeriplaneta americana.We found thatP. americanahosts a diverse core gut microbiome that remains stable after drastic long-term changes in diet. While other insects, notably ant and bee species, have evolved mechanisms for maintaining a stable association with specific gut microbiota, these insects typically host low-diversity gut microbiomes and consume specialized diets. In contrast,P. americanahosts a gut microbiota that is highly species rich and consumes a diverse solid diet, suggesting that cockroaches have evolved unique mechanisms for developing and maintaining a stable gut microbiota.