Identification of Benzo[ a ]pyrene-Metabolizing Bacteria in Forest Soils by Using DNA-Based Stable-Isotope Probing

• DOI: 10.1128/aem.01983-15
• Publication Date: 2015-08-08T02:40:28Z
• Authors: Mengke Song; Chunling Luo; Longfei Jiang; Dayi Zhang; Yujie Wang; Gan Zhang
• Keywords: Stable-isotope probing; Soil microbiology

ABSTRACT DNA-based stable-isotope probing (DNA-SIP) was used in this study to investigate the uncultivated bacteria with benzo[ a ]pyrene (BaP) metabolism capacities two Chinese forest soils (Mt. Maoer Heilongjiang Province and Mt. Baicaowa Hubei Province). We characterized three different phylotypes responsibility for BaP degradation, none of which were previously reported as BaP-degrading microorganisms by SIP. In soil microcosms, putative degraders classified belonging genus Terrimonas (family Chitinophagaceae , order Sphingobacteriales ), whereas Burkholderia spp. key soils. The addition metabolic salicylate significantly increased degradation efficiency soils, BaP-metabolizing shifted family Oxalobacteraceae (genus unclassified). Meanwhile, did not change either or Baicaowa. Polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHD) genes amplified, sequenced, quantified DNA-SIP 13 C heavy fraction further confirm metabolism. By illuminating microbial diversity additive effects on across results our understanding natural attenuation provided possible approach enhance bioremediation BaP-contaminated