Porcine Small and Large Intestinal Microbiota Rapidly Hydrolyze the Masked Mycotoxin Deoxynivalenol-3-Glucoside and Release Deoxynivalenol in Spiked Batch Cultures In Vitro

• DOI: 10.1128/aem.02106-17
• Publication Date: 2017-11-03T16:24:58Z
• Authors: Silvia W. Gratz; Valerie Currie; Anthony J. Richar...; Gary Duncan; Grietje Holtrop; Freda Farquharson; Petra Louis; Philippe Pinton; Isabelle P. Oswald
• Keywords: Cecum; Caecum; Jejunum; Large intestine; Avena

ABSTRACT Mycotoxin contamination of cereal grains causes well-recognized toxicities in animals and humans, but the fate plant-bound masked mycotoxins gut is less well understood. Masked have been found to be stable under conditions prevailing small intestine are rapidly hydrolyzed by fecal microbiota. This study aims assess hydrolysis mycotoxin deoxynivalenol-3-glucoside (DON3Glc) microbiota different regions porcine intestinal tract. Intestinal digesta samples were collected from jejunum, ileum, cecum, colon, feces 5 pigs immediately frozen anaerobic conditions. Sample slurries prepared M2 culture medium, spiked with DON3Glc or free deoxynivalenol (DON; 2 nmol/ml), incubated anaerobically for up 72 h. concentrations determined using liquid chromatography-tandem mass spectrometry, composition was a quantitative PCR methodology. The jejunal very slowly, while efficiently DON3Glc. No further metabolism DON observed any sample. microbial load ileum significantly those distal regions, whereas colon did not differ. IMPORTANCE Results this clearly demonstrate that large pigs. Once released, toxicity absorption tract likely occur vivo . supports need include metabolites risk assessments regulatory actions feed food.