Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glycoprotein 2

• DOI: 10.1128/aem.02279-21
• Publication Date: 2022-01-12T20:16:31Z
• Authors: Christin Bartlitz; Rafał Kolenda; Jarosław Chilimoniuk; Krzysztof Grzymajło; Stefan Rödiger; Rolf Bauerfeind; Aamir Ali; Veronika Tchesnokova; Dirk Roggenbuck; Peter Schierack
• Keywords: Enteropathogenic Escherichia coli; Enterotoxigenic Escherichia coli; Pathogenic Escherichia coli; Fimbriae Proteins

Pathogenic bacteria, such as enteropathogenic Escherichia coli (EPEC) and enterotoxigenic E. (ETEC), cause diarrhea in mammals. In particular, colonizes infects the gastrointestinal tract via type 1 fimbriae (T1F). Here, major zymogen granule membrane glycoprotein 2 (GP2) acts a host cell receptor. GP2 is also secreted by pancreas various mucous glands, interacting with luminal fimbriae-positive coli. It unknown whether isoforms demonstrate specific pathotype binding. this study, we investigated interactions of human, porcine, bovine EPEC ETEC, well commensal isolates GP2. We first defined pathotype- host-associated FimH variants. Second, could prove that bound to variants degrees. However, GP2-FimH did not seem be influenced specificity contrast, soluble affected ETEC infection phagocytosis rates macrophages. Preincubation reduced lines. Furthermore, preincubation improved rate Our findings suggest plays role defense against corresponding immune response. IMPORTANCE Infection pathogenic certain pathotypes, results Pathogens, including zoonotic agents, can infect different hosts or show specificity. There are strains which frequently transmitted between humans animals, whereas other tend colonize only one host. This still fully understood. selective receptor for particular adhesin but selector species-specific group. involved regulation colonization thus represents molecule interest prevention treatment disease.