Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae

• DOI: 10.1128/aem.02319-16
• Publication Date: 2016-10-29T01:30:29Z
• Authors: Zhijia Fang; Zhongxiang Chen; Song Wang; Ping Shi; Yuhu Shen; Youshang Zhang; Junhua Xiao; Zhiwei Huang
• Keywords: Fatty acid desaturase

The heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops humans. Saccharomyces cerevisiae one of most commonly organisms in investigation environmental toxicity. We investigated stress adaptive mechanisms yeast by screening genome-wide essential gene overexpression library. A candidate gene, OLE1, encodes delta-9 desaturase was associated with high anti-cadmium-stress activity. results demonstrated that expression OLE1 positively correlated tolerance induction independent Mga2p Spt23p (important regulatory factors for OLE1). Moreover, response stress, cellular levels monounsaturated fatty acids were increased. addition exogenous unsaturated simulated leading resistance. Such regulation synthesis may serve as positive feedback mechanism help cells counter lipid peroxidation cytoplasmic membrane damage caused cadmium.A S. encoding desaturase, data suggest cadmium. discovery involvement stability indicate novel defense strategy against stress.