The Noncommensal Bacterium Methylococcus capsulatus (Bath) Ameliorates Dextran Sulfate (Sodium Salt)-Induced Ulcerative Colitis by Influencing Mechanisms Essential for Maintenance of the Colonic Barrier Function

• DOI: 10.1128/aem.02464-12
• Publication Date: 2012-10-13T02:03:21Z
• Authors: Charlotte R. Klei...; Lene T. Olsen Hult; Signe Spetalen; Magne Kaldhusdal; Trine Eker Christ...; Oskar Bengtsson; Odd Helge Romarheim; Morten Jacobsen; Tor Lea
• Keywords: 0301 basic medicine; Bacillales; Mucin-2; Colon; Dextran Sulfate; Ralstonia; 6. Clean water; Diet; 3. Good health; Mice, Inbred C57BL; Disease Models, Animal; Mice; 03 medical and health sciences; Methylococcus capsulatus; Animals; Colitis, Ulcerative; Intestinal Mucosa

Dietary inclusion of a bacterial meal has recently been shown to efficiently abolish soybean meal-induced enteritis in Atlantic salmon. The objective this study was investigate whether the diet could abrogate disease development murine model epithelial injury and colitis thus possibly have therapeutic potential human inflammatory bowel disease. C57BL/6N mice were fed ad libitum control or an experimental containing 254 g/kg body weight BioProtein, consisting Methylococcus capsulatus (Bath), together with heterogenic bacteria Ralstonia sp., Brevibacillus agri, Aneurinibacillus sp. At day 8, induced by 3.5% dextran sulfate sodium (DSS) drinking water for 6 days. Symptoms DSS treatment less profound after prophylactic BioProtein. Colitis-associated parameters such as reduced weight, colon shortening, damage also showed significant improvement. Levels acute-phase reactants, proteins whose plasma concentrations increase response inflammation, neutrophil infiltration reduced. On other, increased cell proliferation enhanced mucin 2 (Muc2) transcription indicated improved integrity colonic layer. BioProtein mainly consists (Bath) (88%). results that we obtained when using M. similar those model. Our show noncommensal bacterium attenuate DSS-induced enhancing barrier function, judged Muc2 transcription.