The Dynamin-Like GTPase FgSey1 Plays a Critical Role in Fungal Development and Virulence in Fusarium graminearum

• DOI: 10.1128/aem.02720-19
• Publication Date: 2020-03-26T13:05:48Z
• Authors: Xuefa Chong; Chenyu Wang; Yao Wang; Yixiao Wang; Liyuan Zhang; Yuancun Liang; Lei Chen; Shenshen Zou; Hansong Dong
• Keywords: Fungal Proteins; 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; Fusarium; Virulence; Vesicular Transport Proteins; Lipid Droplets; Trichothecenes; Gene Deletion

Fusarium graminearum, the main pathogenic fungus causing head blight (FHB), produces deoxynivalenol (DON), a key virulence factor, which is synthesized in endoplasmic reticulum (ER). Sey1/atlastin, dynamin-like GTPase protein, known to be required for homotypic fusion of ER membranes, but functions this protein are unknown fungi. Here, we characterized Sey1/atlastin homologue FgSey1 F. graminearum Like located ER. The FgSEY1 deletion mutant exhibited significantly reduced vegetative growth, asexual development, DON biosynthesis, and virulence. Moreover, ΔFgsey1 was impaired formation normal lipid droplets (LDs) toxisomes, both participate biosynthesis. GTPase, helix bundle (HB), transmembrane segment (TM), cytosolic tail (CT) domains essential its function, only TM domain responsible localization. Furthermore, mutants FgSey1K63A FgSey1T87A lacked activity failed rescue defects mutant. Collectively, our data suggest that affects generation LDs toxisomes biosynthesis pathogenesis graminearumIMPORTANCEFusarium major plant pathogen causes (FHB) wheats worldwide. In addition reducing yield, infection also results production (DON) mycotoxins, harmful humans animals therefore cause great economic losses through pollution food products animal feed. At present, effective strategies controlling FHB not available. Therefore, understanding regulation mechanisms fungal pathogenesis, important development control disease. study, demonstrated homologue, FgSey1, production, pathogenicity Our provide novel information on critical roles pathogenicity; therefore, could potential target disease caused by graminearum.