Genus-Specific Primers for Study of Fusarium Communities in Field Samples

• DOI: 10.1128/aem.02748-15
• Publication Date: 2015-10-31T00:24:36Z
• Authors: Ida Karlsson; Véronique Edel-He...; Nadine Gautheron; Mikael Brandström...; Anna-Karin Kolseth; Christian Steinberg; Paula Persson; Hanna Friberg
• Keywords: Primer (cosmetics); Amplicon; Species complex

ABSTRACT Fusarium is a large and diverse genus of fungi great agricultural economic importance, containing many plant pathogens mycotoxin producers. To date, high-throughput sequencing communities has been limited by the lack genus-specific primers targeting regions with high discriminatory power at species level. In present study, we evaluated two -specific primer pairs translation elongation factor 1 (TEF1). We also new pair Fa+7/Ra+6. Mock reflecting phylogenetic diversity were used to evaluate accuracy in relative abundance species. TEF1 amplicons subjected 454 characterize communities. Field samples from soil wheat kernels included test method on more-complex material. For kernel samples, single PCR was sufficient, while for nested necessary. The newly developed Fa+7/Ra+6 Fa/Ra accurately reflected composition mock DNA field 47 operational taxonomic units identified, highest soil. community dominated members incarnatum-Fusarium equiseti complex, contradicting findings previous studies. successfully applied analyze material can facilitate further studies ecology.