Investigation of the malE Promoter and MalR, a Positive Regulator of the Maltose Regulon, for an Improved Expression System in Sulfolobus acidocaldarius

• DOI: 10.1128/aem.03050-13
• Publication Date: 2013-11-23T02:13:18Z
• Authors: Michaela Wagner; Alexander Wagner; Xiaoqing Ma; Julia Christin Kort; Abhrajyoti Ghosh; Bernadette Rauch; Bettina Siebers; Sonja-Verena Albers
• Keywords: Sulfolobus acidocaldarius; Regulon; Maltose-binding protein

In this study, the regulator MalR (Saci_1161) of TrmB family from Sulfolobus acidocaldarius was identified and shown to be involved in transcriptional control maltose regulon (Saci_1660 Saci_1666), including ABC transporter (malEFGK), α-amylase (amyA), α-glycosidase (malA). The ΔmalR deletion mutant exhibited a significantly decreased growth rate on dextrin but not sucrose. expression genes organized induced only presence medium, indicating that MalR, contrast its TrmB-like homologues, is an activator gene cluster. Electrophoretic mobility shift assays revealed binding malE independent sugars. Here we report identification archaeal protein which acts as controls transport metabolic conversion S. acidocaldarius, use for improvement system under optimized (malE) promoter by mutagenesis.