Expression in Escherichia coli of the Native cyt1Aa from Bacillus thuringiensis subsp. israelensis

• DOI: 10.1128/aem.03068-09
• Publication Date: 2010-03-27T02:54:25Z
• Authors: Vladislav Sazhenskiy; Arieh Zaritsky; Mark Itsko
• Keywords: Bacillus thuringiensis; Bacillaceae

The gene cyt1Aa is one of the genes in complex determining mosquito larvicidity Bacillus thuringiensis subsp. israelensis. Previous cloning Escherichia coli resulted a 48-bp addition upstream, encoding chimera. Here, was recloned without artifact, and its toxicity against Aedes aegypti larvae host E. cells retested.