Population Structure and Abundance of Arsenite-Oxidizing Bacteria along an Arsenic Pollution Gradient in Waters of the Upper Isle River Basin, France

• DOI: 10.1128/aem.03104-09
• Publication Date: 2010-05-08T02:25:30Z
• Authors: Marianne Quéméneur; Aurélie Cébron; Patrick Billard; Fabienne Battagli...; Francis Garrido; Corinne Leyval; Catherine Joulian
• Keywords: Oxidizing agent

Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qPCR) were successfully developed to monitor functional aoxB genes as markers of aerobic arsenite oxidizers. DGGE profiles showed a shift in the structure aoxB-carrying bacterial population, composed members Alpha-, Beta- Gammaproteobacteria, depending on arsenic (As) E(h) levels Upper Isle River Basin waters. The highest gene densities found most As-polluted oxic surface waters but without any significant correlation with environmental factors. Arsenite oxidizers seem play key role As mobility As-impacted